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1st Semester

Advanced Biochemistry

  • Membranes – signal transduction. Signaling pathways coupled with G proteins.
    • cΑΜΡ pathway, protein kinase Α.
    • cΑΜΡ receptors (Epac), activation protein kinase Β (Akt).
    • Phosphoinisitide pathway, protein kinase C.
    • Ca2+ signalling
    • Tyrosine kinases, small G proteins, ΡΙ-3 kinase, protein kinase B activation (Akt).
    • ΜΑΡ kinases ( ERK1,2, JNKs, p38).
    • ΝΟ signaling pathways, cGMP, protein kinase G.
    • Lateral compartmentalization and function of the cytoplasmic membrane: eisosomes and transmembrane transporters
    • Transcription factors (CREB, CREM, NF-kB, AP-1, STAT) and their activation.
    • Prostaglandins.
    • Steroid hormones.
    • Signaling pathways of main cytokines and growth factors, IL-1, TNF-α, TGF-β (SMAD proteins), PDGF, EGF, FGF.
  • Interaction of ECM and cells.
  • Integration of metabolism in prokaryotes and eukaryotes. Control mechanisms of metabolism of carbohydrates, proteins and fats.
  • Protein Structure - Experimental and Theoretical Approaches.


Biochemical Analysis – Clinical Biochemistry

  • Liquid chromatography (gel, ion-exchange, affinity). Theory, applications.
  • HPLC: Theory, techniques, applications. GC: Applications. SFC: Theory, applications.
  • Electrophoresis: Theory and techniques. Capillary electrophoresis: Applications.
  • Enzyme in analysis: Chemistry and applications of enzymes, biosensors.
  • Radionucleids in analysis.
  • Immunoenzymatic analytical methods: Theory, techniques, applications.
  • Automatisation in analysis, Modern auto-analyzers,
  • Methods selection criteria - Development of analytical methods.
  • Selection of methods for the analysis of biological fluids.
  • Basic Physiology (nervous system, liver, gallbladder, pancreas).
  • Biochemistry of the main organs and endocrine glands.
  • Control of organs and endocrine glands functions.
  • Evaluation of results.
  • Analysis of metabolites, drugs and toxic substances.
  • Chemical ecology: Applications of bioactive secondary metabolites of natural origin.
  • Methods of analysis and study of proteins, lipids and carbohydrates.


Literature Review and Research Methodology

  • Searching, recording and presenting the bibliography related to the postgraduate diploma thesis.
  • A series of lectures on contemporary issues of specialization and issues related to the development of communication skills and IT skills.
  • Bibliographic overview of the research field of the subject of Postgraduate Diploma Thesis and its public presentation.

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